PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G54320.2
Common NameASML1, ATWRI1, WRI, WRI1
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family ERF
Protein Properties Length: 356aa    MW: 40598.6 Da    PI: 4.2054
Description ERF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G54320.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1AP253.27.3e-1792144156
          AP2   1 sgykGVrwdkkrgrWvAeIrd.psengkrkrfslgkfgtaeeAakaaiaarkklege 56 
                  s+y+GV +++ +grW+A+I   +   g +k+ +lg++ t eeAa+a++ a+ +++g+
  AT3G54320.2  92 SKYRGVARHHHNGRWEARIGRvF---G-NKYLYLGTYNTQEEAAAAYDMAAIEYRGA 144
                  89****99**********99966...3.6*************************995 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5103211.548157IPR001471AP2/ERF domain
SMARTSM003800.0222163IPR001471AP2/ERF domain
SuperFamilySSF541713.07E-62358IPR016177DNA-binding domain
Gene3DG3DSA:3.30.730.103.7E-62958IPR001471AP2/ERF domain
PfamPF008472.8E-1292143IPR001471AP2/ERF domain
SuperFamilySSF541714.25E-1892153IPR016177DNA-binding domain
CDDcd000181.33E-1692153No hitNo description
SMARTSM003806.7E-2593157IPR001471AP2/ERF domain
PROSITE profilePS5103218.37393151IPR001471AP2/ERF domain
Gene3DG3DSA:3.30.730.102.5E-1893151IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006110Biological Processregulation of glycolytic process
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009744Biological Processresponse to sucrose
GO:0009873Biological Processethylene-activated signaling pathway
GO:0019432Biological Processtriglyceride biosynthetic process
GO:1901959Biological Processpositive regulation of cutin biosynthetic process
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000084anatomyplant sperm cell
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009001anatomyfruit
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001170developmental stageseed development stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 356 aa     Download sequence    Send to blast
MDWEIRGSSL GQKLLEFDSE QERQTRFRAY DSEEAAAHTY DLAALKYWGP DTILNFPAET  60
YTKELEEMQR VTKEEYLASL RRQSSGFSRG VSKYRGVARH HHNGRWEARI GRVFGNKYLY  120
LGTYNTQEEA AAAYDMAAIE YRGANAVTNF DISNYIDRLK KKGVFPFPVN QANHQEGILV  180
EAKQEVETRE AKEEPREEVK QQYVEEPPQE EEEKEEEKAE QQEAEIVGYS EEAAVVNCCI  240
DSSTIMEMDR CGDNNELAWN FCMMDTGFSP FLTDQNLANE NPIEYPELFN ELAFEDNIDF  300
MFDDGKHECL NLENLDCCVV GRESPPSSSS PLSCLSTDSA SSTTTTTTSV SCNYLV
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.351160.0flower| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible251892_at0.0
Expression AtlasAT3G54320-
AtGenExpressAT3G54320-
ATTED-IIAT3G54320-
Functional Description ? help Back to Top
Source Description
TAIRWRINKLED1 encodes transcription factor of the AP2/ERWEBP class. Protein has two plant-specific (AP2/EREB) DNA-binding domains and is involved in the control of storage compound biosynthesis in Arabidopsis. Mutants have wrinkled seed phenotype, due to a defect in the incorporation of sucrose and glucose into triacylglycerols. Transgenic sGsL plants (21-day-old) grown on 6% sucrose for 24 hours had 2-fold increase in levels of expressions (sGsL line carries a single copy of T-DNA containing the Spomin::GUS-Spomin::LUC dual reporter genes in the upper arm of chromosome 5 of ecotype Col-0. The sporamin .minimal. promoter directs sugar-inducible expression of the LUC and GUS reporters in leaves). Regulation by LEC2 promotes fatty acid accumulation during seed maturation.
Function -- GeneRIF ? help Back to Top
  1. The putative AP2/EREBP transcription factor WRINKLED1 (WRI1) is involved in the regulation of seed storage metabolism in Arabidopsis.
    [PMID: 15500472]
  2. ASML1/WRI1 is a transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds
    [PMID: 15753106]
  3. WRI1 is a target of LEAFY COTYLEDON2 and is necessary for the regulatory action on fatty acid metabolism.
    [PMID: 17419836]
  4. WRI1 promotes the flow of carbon to oil during seed maturation by directly activating genes involved in fatty acid synthesis and controlling genes for assembly and storage of triacylglycerol.
    [PMID: 19594710]
  5. WRI1 is able to regulate in planta the activity of the BCCP2 and PKp-beta1 promoters to enhance the transcription level of glycolytic and fatty acid biosynthetic genes.
    [PMID: 19719479]
  6. directly controls AtGLB1 expression
    [PMID: 21070409]
  7. WRI1 activates fatty acid biosynthesis in seeds for triacylglycerol production. WRI1, WRI3 and WRI4 are required in floral tissues to provide acyl chains for cutin synthesis and prevent adherence of these developing organs and subsequent semisterility.
    [PMID: 23243127]
  8. Data show that CUL3 and BPM proteins assemble in planta with WRI1.
    [PMID: 23792371]
  9. the structure and function of the WRI1 plant transcription factor, was investigated.
    [PMID: 23922666]
  10. A C-terminal intrinsically disordered region of WRINKLED1 is important for protein stability.WRINKLED1 plays role in oil accumulation.
    [PMID: 26305482]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT3G54320.2
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT1G21970 (A), AT1G28300 (A)
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT2G40170(R), AT2G43360(A), AT3G22960(A), AT3G24650(R), AT5G15530(A), AT5G49190(A), AT5G52920(A)
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDabscisic acid
Interaction ? help Back to Top
Source Intact With
BioGRIDAT3G54320
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G54320
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY2540380.0AY254038.2 Arabidopsis thaliana WRINKLED1 (WRI1) mRNA, complete cds.
GenBankAY8852450.0AY885245.1 Arabidopsis thaliana activator of sporamin LUC 1 (ASL1) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_974430.10.0ethylene-responsive transcription factor WRI1
SwissprotQ6X5Y60.0WRI1_ARATH; Ethylene-responsive transcription factor WRI1
TrEMBLA8MS570.0A8MS57_ARATH; Ethylene-responsive transcription factor WRI1
STRINGAT3G54320.10.0(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Krizek BA,Prost V,Macias A
    AINTEGUMENTA promotes petal identity and acts as a negative regulator of AGAMOUS.
    Plant Cell, 2000. 12(8): p. 1357-66
    [PMID:10948255]
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  3. Ruuska SA,Girke T,Benning C,Ohlrogge JB
    Contrapuntal networks of gene expression during Arabidopsis seed filling.
    Plant Cell, 2002. 14(6): p. 1191-206
    [PMID:12084821]
  4. Cernac A,Benning C
    WRINKLED1 encodes an AP2/EREB domain protein involved in the control of storage compound biosynthesis in Arabidopsis.
    Plant J., 2004. 40(4): p. 575-85
    [PMID:15500472]
  5. Masaki T, et al.
    ACTIVATOR of Spomin::LUC1/WRINKLED1 of Arabidopsis thaliana transactivates sugar-inducible promoters.
    Plant Cell Physiol., 2005. 46(4): p. 547-56
    [PMID:15753106]
  6. Baud S,Graham IA
    A spatiotemporal analysis of enzymatic activities associated with carbon metabolism in wild-type and mutant embryos of Arabidopsis using in situ histochemistry.
    Plant J., 2006. 46(1): p. 155-69
    [PMID:16553903]
  7. Cernac A,Andre C,Hoffmann-Benning S,Benning C
    WRI1 is required for seed germination and seedling establishment.
    Plant Physiol., 2006. 141(2): p. 745-57
    [PMID:16632590]
  8. Baud S, et al.
    WRINKLED1 specifies the regulatory action of LEAFY COTYLEDON2 towards fatty acid metabolism during seed maturation in Arabidopsis.
    Plant J., 2007. 50(5): p. 825-38
    [PMID:17419836]
  9. Baud S, et al.
    Function of plastidial pyruvate kinases in seeds of Arabidopsis thaliana.
    Plant J., 2007. 52(3): p. 405-19
    [PMID:17892448]
  10. Santos-Mendoza M, et al.
    Deciphering gene regulatory networks that control seed development and maturation in Arabidopsis.
    Plant J., 2008. 54(4): p. 608-20
    [PMID:18476867]
  11. Mu J, et al.
    LEAFY COTYLEDON1 is a key regulator of fatty acid biosynthesis in Arabidopsis.
    Plant Physiol., 2008. 148(2): p. 1042-54
    [PMID:18689444]
  12. Baud S,Lepiniec L
    Regulation of de novo fatty acid synthesis in maturing oilseeds of Arabidopsis.
    Plant Physiol. Biochem., 2009. 47(6): p. 448-55
    [PMID:19136270]
  13. Chen M, et al.
    System analysis of an Arabidopsis mutant altered in de novo fatty acid synthesis reveals diverse changes in seed composition and metabolism.
    Plant Physiol., 2009. 150(1): p. 27-41
    [PMID:19279196]
  14. Huang Y, et al.
    Probing the endosperm gene expression landscape in Brassica napus.
    BMC Genomics, 2009. 10: p. 256
    [PMID:19490642]
  15. Maeo K, et al.
    An AP2-type transcription factor, WRINKLED1, of Arabidopsis thaliana binds to the AW-box sequence conserved among proximal upstream regions of genes involved in fatty acid synthesis.
    Plant J., 2009. 60(3): p. 476-87
    [PMID:19594710]
  16. He YQ,Wu Y
    Oil body biogenesis during Brassica napus embryogenesis.
    J Integr Plant Biol, 2009. 51(8): p. 792-9
    [PMID:19686376]
  17. Baud S,Wuillème S,To A,Rochat C,Lepiniec L
    Role of WRINKLED1 in the transcriptional regulation of glycolytic and fatty acid biosynthetic genes in Arabidopsis.
    Plant J., 2009. 60(6): p. 933-47
    [PMID:19719479]
  18. Lonien J,Schwender J
    Analysis of metabolic flux phenotypes for two Arabidopsis mutants with severe impairment in seed storage lipid synthesis.
    Plant Physiol., 2009. 151(3): p. 1617-34
    [PMID:19755540]
  19. Liu J, et al.
    Increasing seed mass and oil content in transgenic Arabidopsis by the overexpression of wri1-like gene from Brassica napus.
    Plant Physiol. Biochem., 2010. 48(1): p. 9-15
    [PMID:19828328]
  20. Li J,Yu M,Geng LL,Zhao J
    The fasciclin-like arabinogalactan protein gene, FLA3, is involved in microspore development of Arabidopsis.
    Plant J., 2010. 64(3): p. 482-97
    [PMID:20807209]
  21. Baud S, et al.
    PII is induced by WRINKLED1 and fine-tunes fatty acid composition in seeds of Arabidopsis thaliana.
    Plant J., 2010. 64(2): p. 291-303
    [PMID:21070409]
  22. Pouvreau B, et al.
    Duplicate maize Wrinkled1 transcription factors activate target genes involved in seed oil biosynthesis.
    Plant Physiol., 2011. 156(2): p. 674-86
    [PMID:21474435]
  23. Moreno-P
    Reduced expression of FatA thioesterases in Arabidopsis affects the oil content and fatty acid composition of the seeds.
    Planta, 2012. 235(3): p. 629-39
    [PMID:22002626]
  24. Durrett TP,Weise SE,Benning C
    Increasing the energy density of vegetative tissues by diverting carbon from starch to oil biosynthesis in transgenic Arabidopsis.
    Plant Biotechnol. J., 2011. 9(8): p. 874-83
    [PMID:22003502]
  25. Andriotis VM, et al.
    Altered starch turnover in the maternal plant has major effects on Arabidopsis fruit growth and seed composition.
    Plant Physiol., 2012. 160(3): p. 1175-86
    [PMID:22942388]
  26. To A, et al.
    WRINKLED transcription factors orchestrate tissue-specific regulation of fatty acid biosynthesis in Arabidopsis.
    Plant Cell, 2012. 24(12): p. 5007-23
    [PMID:23243127]
  27. Li-Beisson Y, et al.
    Acyl-lipid metabolism.
    Arabidopsis Book, 2013. 11: p. e0161
    [PMID:23505340]
  28. Fukuda N,Ikawa Y,Aoyagi T,Kozaki A
    Expression of the genes coding for plastidic acetyl-CoA carboxylase subunits is regulated by a location-sensitive transcription factor binding site.
    Plant Mol. Biol., 2013. 82(4-5): p. 473-83
    [PMID:23733600]
  29. Chen L, et al.
    Arabidopsis BPM proteins function as substrate adaptors to a cullin3-based E3 ligase to affect fatty acid metabolism in plants.
    Plant Cell, 2013. 25(6): p. 2253-64
    [PMID:23792371]
  30. Ma W, et al.
    Wrinkled1, a ubiquitous regulator in oil accumulating tissues from Arabidopsis embryos to oil palm mesocarp.
    PLoS ONE, 2013. 8(7): p. e68887
    [PMID:23922666]
  31. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  32. Kilaru A, et al.
    Oil biosynthesis in a basal angiosperm: transcriptome analysis of Persea Americana mesocarp.
    BMC Plant Biol., 2015. 15: p. 203
    [PMID:26276496]
  33. Ma W, et al.
    Deletion of a C-terminal intrinsically disordered region of WRINKLED1 affects its stability and enhances oil accumulation in Arabidopsis.
    Plant J., 2015. 83(5): p. 864-74
    [PMID:26305482]
  34. Focks N,Benning C
    wrinkled1: A novel, low-seed-oil mutant of Arabidopsis with a deficiency in the seed-specific regulation of carbohydrate metabolism.
    Plant Physiol., 1998. 118(1): p. 91-101
    [PMID:9733529]